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. 1999 Jan;73(1):198–204. doi: 10.1128/jvi.73.1.198-204.1999

FIG. 3.

FIG. 3

RdRp recognition of key nucleotides is required even in the presence of a nucleotide insertion. (A) The sequence of the WT proscript is presented as described in the legend to Fig. 2A. The identities of the insertions between nt −13 and −12 are shown in the area below the gap in the WT sequence. Additional changes at specific positions are indicated by the letters in the dashed lines. (B) Autoradiograph of RdRp products synthesized from proscripts containing insertions and substitutions within the −13/−11 region of the subgenomic promoter analyzed by 20% denaturing PAGE. Duplicate independent reactions are shown. Positions of the 14- and 13-nt products are indicated on the left.