FIG. 4.

Nucleotide insertions and deletions within the −11/+1 region. (A) The sequence of the WT proscript is presented as described in the legend to Fig. 2A. Changes from the WT sequence between nt −4 and −3 are illustrated in the area below the gap in the sequence. An empty box indicate that a nucleotide has been deleted. The RdRp products generated by each proscript and the values for 1 standard deviation from the mean are listed on the right. All values represent at least six independent assays. Quantified values in reactions with multiple products represent the sum of all products made. (B) Autoradiograph of RdRp products synthesized from proscripts containing insertions and deletions within the −11/+1 region of the subgenomic promoter. Duplicate independent reactions are shown, and positions of the 14- and 13-nt products are indicated on the left. Anomalous-size products are clearly visible as bands of greater than 14 nt in lanes 7, 8, 15, and 16.