TABLE 1.
Insertions and deletions of nucleotides between positions −17 and −11
| Proscript | % of WT | Sequence (3′-5′)a |
|---|---|---|
| WT | 100 | cuaGauACaGgauuaagucgCau |
| Mutant | ||
| −17/−14 | ||
| A-INS −17/−16 | 12 ± 4 | cuaGAauACaGgauuaagucgCau |
| C-INS −17/−16 | 10 ± 4 | cuaGCauACaGgauuaagucgCau |
| U-INS −17/−16 | 0 ± 0 | cuaGUauACaGgauuaagucgCau |
| AA-INS −17/−16 | 1 ± 1 | cuaGAAauACaGgauuaagucgCau |
| DEL −16 | 1 ± 1 | cuaG*uACaGgauuaagucgCau |
| A-INS −17/−16, −17 G to U | 1 ± 1 | cuaUAauACaGgauuaagucgCau |
| −14 to −13 | ||
| A-INS −14/−13 | 25 ± 8 | cuaGauAaCaGgauuaagucgCau |
| U-INS −14/−13 | 4 ± 3 | cuaGauAuCaGgauuaagucgCau |
| AA-INS −14/−13 | 10 ± 4 | cuaGauAaaCaggauuaaguccCau |
| −13 to −11 | ||
| A-INS −13/−12 | 36 ± 10 | cuaGauACaaGgauuaagucgcau |
| U-INS −13/−12 | 41 ± 21 | cuaGauACuaGgauuaagucgcau |
| AA-INS −13/−12 | 11 ± 5 | cuaGauACaaaGgauuaagucgcau |
| UU-INS −13/−12 | 15 ± 4 | cuaGauACuuaGgauuaagucgcau |
| DEL −12 | 27 ± 7 | cuaGauAC*Ggauuaagucgcau |
a
Nucleotides inserted are indicated in boldface, letters in larger font size represent those previously found to be required for recognition by RdRp, and asterisks denote deleted nucleotides.