TABLE 2.
Insertions and deletions of nucleotides between positions −11 and +1
| Proscript | % of WT | Sequence (3′-5′)a |
|---|---|---|
| WT | 100 | cuaGauACaGgauuaagucgcau |
| Mutant | ||
| U-INS −11/−10 | 69 ± 15 | cuaGauACaGugauuaagucgcau |
| UU-INS −11/10 | 52 ± 18 | cuaGauACaGuugauuaagucgcau |
| DEL −10 | 65 ± 7 | cuaGauACaG*auuaagucgcau |
| DEL −10,−9 | 24 ± 19 | cuaGauACaG**uuaagucgcau |
| DEL −8 | 71 ± 15 | cuaGauACaGga*uaagucgcau |
| Del −8,−7 | 23 ± 9 | cuaGauACaGga**aagucgcau |
| U-INS −7/−6 | 114 ± 15 | cuaGauACaGgauuuaagucgcau |
| UU-Ins −7/−6 | 59 ± 17 | cuaGauACaGgauuuuaagucgcau |
| A-INS −4/−3 | 43 ± 14 | cuaGauACaGgauuaagaucgcau |
| U-INS −4/−3 | 61 ± 13 | cuaGauACaGgauuaaguucgcau |
| AA-INS −4/−3 | 70 ± 19 | cuaGauACaGgauuaagaaucgcau |
| UU-INS −4/−3 | 15 ± 2 | cuaGauACaGgauuaaguuucgcau |
| 4U-INS −4/−3 | 26 ± 18 | cuaGauACaGgauuaaguuuuucgcau |
| DEL −4 | 45 ± 15 | cuaGauACaGgauuaa*ucgcau |
| DEL −4,−3 | 5 ± 4 | cuaGauACaGgauuaa**cgcau |
a
Nucleotides inserted are indicated in boldface, letters in larger font size denote those previously found to be required for recognition by RdRp, and asterisks denote deleted nucleotides.