Table 5.
System suitability and validation parameters for the proposed HPLC–UV method
| Parameters | HPLC | USP Reference values |
|---|---|---|
| Linearity (µg/mL) | 1–200 | |
| Correlation coefficient (r) | 0.9999 | |
| Slope | 2.291 | |
| Intercept | 0.7938 | |
| SD of residuals from line | 0.2201 | |
| LOD (µg/mL) | 0.317 | |
| LOQ (µg/mL) | 0.961 | |
| Accuracy (recovery % ± SD) | 101.34 ± 0.88 | |
| Precision (intraday) | 99.65 ± 1.65 | |
| Precision (inter-day) | 101.45 ± 1.98 | |
| Extraction recovery % | 7.554 | |
| Matrix effect | 8.423 | |
| tR, min | 9.18 ± 0.05 | |
| Tailing factor (T) | 1.04 | T ≤ 2, T = 1 for symmetric peak |
| Capacity factor (K′) | 2.11 | K' = 1–10 acceptable |
| Plates number (N) | 5884 | N > 2000 |
| Height equivalent to theoretical plate (HETP) | 0.06 | The smaller the value, the higher the column efficiency |
| Experimental resolution (Rs)** | 2.08 | Rs ≥ 2 |
**Experimental resolution between VA and internal standard