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. 2023 Jul 28;9:78. doi: 10.1038/s41421-023-00581-9

Fig. 7. BANAL-20-52 and BANAL-20-236 S pseudovirions utilize a broad range of animal ACE2 orthologs for virus entry.

Fig. 7

a Western blot analysis of the cell lysates of HEK293 cells transiently over-expressing FLAG-tagged animal ACE2 orthologs using mouse monoclonal anti-FLAG M2 antibodies. β-actin served as loading controls. b Transduction of HEK293 cells expressing different animal ACE2 orthologs by SARS-CoV S, SARS-CoV-2 S, BANAL-20-52 S, and BANAL-20-236 S pseudovirions. The experiments were done in triplicate and repeated at least three times. One representative is shown with error bars indicating SD. c, d Transduction of 293 cells expressing tree shrew ACE2 or guinea pig ACE2 by WT and Q498H mutant SARS-CoV-2 S pseudovirions (c), and by WT and H498Q mutant BANAL-20-52 S pseudovirions (d). Data are represented as mean ± SD from at least triplicates. P-values in c and d are calculated by unpaired two-sided Student’s t-test. *P < 0.05; **P < 0.01; ns, P > 0.05.