Table 1.
Structural features of antibodies in this study, and helical parameters of Fab-rsCSP cryo-EM structures
| CDR3 Length (aa) | SHM (# aa) | Helical Parameters | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mAb | HV Allele | KV/LV Allele | HC | LC | HC | LC | Full epitope (cryo-EM) | Map res. (Å) | Fabs bound | Turn (°) | Fabs/ turn | Rise (Å) | Pitch (Å) | Diam (Å) |
| 227 | 3–33*01 | LV1–40*01 | 15 | 11 | 10 | 3 | ANPNANPNA | 3.3 | 8 | 69.9 | 5.2 | 2.3 | 12 | 35 |
| 311 | 3–33*01 | LV1–40*01 | 12 | 12 | 10 | 5 | ANPNANPNA | 3.0 | 11 | 68 | 5.3 | 10.6 | 50 | 27 |
| 239 | 3–33*03 | KV1–5*05 | 12 | 10 | 11 | 9 | NpnANPNANPNA | 3.72 | 10 | 71.9 | 5 | 14.4 | 72 | 25 |
| 334 | 3–33*08 | KV1–5*05 | 14 | 10 | 10 | 6 | NpnANPNANPNA | 3.62 | 9 | 80 | 4.5 | 9.3 | 42 | 19 |
| 364 | 3–33*03 | KV1–5*05 | 10 | 8 | 10 | 8 | ANPNANPNA | 3.84 | 5 | 61.7 | 5.8 | 1.7 | 10 | 35 |
| 337 | 3–33*08 | KV3–15*01 | 15 | 8 | 12 | 4 | ANPNANPNANP | 2.7 | 7 | 83.8 | 4.3 | 8.6 | 37 | 19 |
| 356 | 3–33*03 | KV3–15*01 | 15 | 8 | 12 | 8 | ANPNANPNANP | 3.2 | 11 | 68 | 5.3 | 10.6 | 50 | 27 |
Germline alleles were derived from the IMGT database, and CDR lengths are according to IMGT definitions. Full epitope describes the complete epitope of one Fab in the rsCSP cryo-EM structure. The core NPNA2 epitope is in bold. Map res. is the overall resolution of reconstruction. The helical parameters were calculated from measurements in UCSF-Chimera. Helical turn: the angular step between adjacent Fabs on central rsCSP helix, as measured from the center of the helix. Helical pitch: the length required to complete one full helical turn, measured parallel to the rsCSP helical axis. Helical rise: the distance traversed along the rsCSP helix by each Fab, measured parallel to helical axis.