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. 2023 Jul 28;13:12231. doi: 10.1038/s41598-023-39210-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antigen-specific antibody production of bone marrow and spleen cells from the immunized mice. Bone marrow (A–C) and spleen cells (D–F) were harvested at day 7 post virus infection and cultured in iPR8-coated plates for 1 day or 7 days, respectively. IgG, IgG1, and IgG2a ELISA was performed to measure the antigen-specific antibody production. All results are expressed as mean ± standard deviation (SD) with individual dots. Statistical analysis was performed using one-way ANOVA and Tukey’s post-multiple comparison tests. *P < 0.0332, **P < 0.0021, ***P < 0.0002, ****P < 0.0001.