Table 2.
Cross-platform analysis of the multiplex qPCR-HRM assay.
| Pathogen | Real-time PCR machines with Tm values | ||
|---|---|---|---|
| CFX 96 (BioRad) | LC480ll (Roche) | QS6 (Life technologies) | |
| Brucella spp. | 83 ± 0.6 (82.4–83.6) | 83.33 ± 0.44(82.89–83.77) | 83.48 ± 0.79 (82.69–84.27) |
| L. monocytogenes | 77.4 ± 0.4 (77.0–77.8) | 77.69 ± 0.45 (77.24–78.14) | 77.98 ± 0.5 (77.48–78.48) |
| C. burnetii | 80.4 ± 0.6 (79.8–81.0) | 80.82 ± 0.46 (80.36–81.28) | 80.97 ± 0.5 (80.47–81.47) |
| Leptospira spp. | 75.5 ± 0.5 (75.0–76.0) | 75.99 ± 0.48 (75.51–76.47) | 76.24 ± 0.5 (75.74–76.74) |
Different real-time PCR instruments were used for assay evaluation with their respective amplicon melting temperature values indicated for the targeted four bacterial pathogens.