KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| Rabbit anti-Nova1 | Abcam | Cat# ab183024; RRID:AB_2632587 |
| Mouse anti-Impact | Abcam | Cat# ab72444; RRID:AB_1269162 |
| Rabbit anti-Impact | Thermo Fisher Scientific | Cat# PA5–59805; RRID:AB_2642711 |
| Mouse anti-hCG alpha | Nnovus biochlogicals | Cat# NBP2–52442 |
| Goat anti-Doublecortin | Santa cruz | Cat# sc-271390; RRID:AB_10610966 |
| Chicken anti-Map2 | Novus biological | Cat# NB300–213; RRID:AB_2138178 |
| Human anti-pan NOVA | Yang et al.4 | N/A |
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| Bacterial and virus strains | ||
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| pUltra | Addgene | Plasmid #24129 |
| pUltra-Cre | This study | N/A |
| pUltra-mNova1 | This study | N/A |
| pUltra-mNova1-ex4 | This study | N/A |
| pUltra-Cre-Impact | This study | N/A |
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| Critical commercial assays | ||
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| Dual-Luciferase Reporter Assay System | Promega | E1910 |
| Dynabeads mRNA Purification Kit | Ambion | Cat# 61006 |
| Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) | Illumina | Cat# MRZH116 |
| TruSeq RNA Sample Preparation Kit v2 | Illumina | Cat# RSS-122–2001 |
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| Deposited data | ||
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| RNA-seq | This paper | GEO: GSE212473 |
| NOVA1-CLIP | This paper | GEO: GSE212547 |
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| Experimental models: Cell lines | ||
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| Neuro-2A | ATCC | CCL-131 |
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| Experimental models: Organisms/Strains | ||
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| Mouse: cKO: GAD2cre-Nova1f/f | This paper | N/A |
| Mouse: cHet: GAD2cre-Nova1f/w | This paper | N/A |
| Mouse: conditional Nova1 KO: Nova1f/f | This paper | N/A |
| Mouse: GAD2-IRES-Cre: B6N.Cg-Gad2tm2(cre)Zjh/J | The Jackson Labpratory | Strain# 019022 |
| Mouse: Nova1 KO: Nova1 knock out | Robert B. Darnell Lab. | Neuron. 2000 Feb; 25(2):359–71. https://doi.org/10.1016/s0896-6273(0080900-9)). |
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| Oligonucleotides | ||
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| siGENOME Mouse Nova1 siRNA SMARTpool | horizon | M-161826-00–0005 |
| siGENOME Non-Targeting siRNA Pool #1 | horizon | D-001206-13-05 |
| qPCR, mNova1, Fwd: 5′-catctgaccccatgaccac-3′ | This paper | N/A |
| qPCR, mNova1, Rvs: 5′-tgctgtgctgttgggaacta-3′ | This paper | N/A |
| qPCR, mImpact, Fwd: 5′-ccttcttgcaggactgtgaa-3′ | This paper | N/A |
| qPCR, mImpact, Rvs: 5′-caccaccaccatgacattct-3′ | This paper | N/A |
| qPCR, mGapdh, Fwd: 5′-aagagggatgctgcccttac-3′ | This paper | N/A |
| qPCR, mGapdh, Rvs: 5′-ccattttgtctacgggacga-3′ | This paper | N/A |
|
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| Recombinant DNA | ||
|
| ||
| pcDNA3.1(+) | Thermo Fisher | Cat# V79020 |
| pcDNA3.1(+)-mNova1 | This paper | N/A |
| pcDNA3.1(+)-mNova1-ex4 | This study | N/A |
| pmirGLO Dual-Luciferase miRNA Target Expression Vector | Promega | Cat# E1330 |
| pmirGLO-Impact 3′UTR | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak1 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak2 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak3 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak1&2 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak1&3 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak2&3 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆peak1&2&3 | This study | N/A |
| pmirGLO-Impact 3′UTR_∆p1a | This study | N/A |
| pmirGLO-Impact 3′UTR_miRNAmut | This study | N/A |
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| Software and algorithms | ||
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| CLIP Tool Kit (CTK) | Zhang lab | https://zhanglab.c2b2.columbia.edu/index.php/CTK_Documentation |
| OLego | Zhang lab | https://zhanglab.c2b2.columbia.edu/index.php/OLego_Documentation |
| Quantas | Zhang lab | https://zhanglab.c2b2.columbia.edu/index.php/Quantas_Documentation |
| edgeR | Robinson et al.65 | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| IGV (2.13.0) | Thorvaldsdottir et al.50 | https://software.broadinstitute.org/software/igv/2.13.x |