Table 1.
Additional Rpb7 mutants
| Mutant | Residue mutated/deleted | Mutant location | Mutant interaction with | Fold UV sensitivity and level of TCR enhancementa |
||||
|---|---|---|---|---|---|---|---|---|
| WT | rad7Δ | rad26Δ rad7Δ | spt4Δ rad26Δ rad7Δ | rad14Δ | ||||
| RNP-1 | 15–18Δ | RNP (K1/loop) | core_RNAPII | N | N | 3 (2) | 5 (2) | N |
| RNP-2 | K5S F18A R21W | RNP (A1, K1-loop) | core_RNAPII, Rpb4 | N | 10 | 5 (1) | N (0) | N |
| RNP-3 | G66I | RNP (tip loop) | core_RNAPII | - | - | N | N | - |
| RNP-4 | 63–67Δ | RNP (tip loop) | core_RNAPII | N | 5 | 3 (2) | lethal | 10 |
| RNP-5 | K41D 63–67Δ | RNP (tip loop, A2-A3 linker) | core_RNAPII | 5 | 5 | 10 (2) | lethal | 5 |
| RNP-6 | Y44R L62Q N71G | RNP (tip loop, A3) | core_RNAPII | - | N | N | N | - |
| RNP-7 | 65–90Δ | RNP (tip loop, A4); OB (B1, B1′) | core_RNAPII, Rpb4, Spt5_KOW3 | lethal | lethal | lethal | lethal | lethal |
| RNP-8 | K5S | RNP (A1) | Rpb4 | - | N | N | N | - |
| RNP-9 | I4A D6A L7A L9A | RNP (A1) | Rpb4 | - | N | N | N | - |
| RNP-10 | F2A F3A I4A K5S | RNP (A1) | Rpb4 | lethal | lethal | lethal | lethal | lethal |
| RNP-11 | D6A L26V L30V K41G | RNP (A1, K2, A2-A3 linker) | Rpb4, core_RNAPII | - | N | N | N | - |
| RNP-12 | E33R E35R K41D | RNP (K2, A2-A3 linker) | Rpb4 | N | N | N (1) | N (1) | N |
| OB-1 | V86A D88K T90A | OB (B1) | Rpb4 | N | N | N (1) | N | N |
| OB-2 | V86A D88K T90A R142A I143D R144A | OB (B1, B4) | Rpb4 | - | 10 | 3 | lethal | lethal |
| OB-3 | V86A D88K T90A R142A I143D R144A 167–171Δ | OB (B1, B4, B6) | Rpb4 | - | 20 | 5 (1) | lethal | lethal |
| OB-4 | 140–144Δ | OB (C3-B4 linker, B4) | Rpb4 | - | 10 | 10 | lethal | lethal |
| OB-5 | K140A R142A I143D R144A K146A | OB (C3-B4 linker, B4) | Rpb4 | lethal | lethal | lethal | lethal | lethal |
| OB-6 | R142A I143D R144A | OB (B4) | Rpb4 | N | 5 | 5 (0) | 20 (2) | lethal |
| OB-7 | 142–144Δ | OB (B4) | Rpb4 | - | 5 | 3 | lethal | lethal |
| OB-8 | R142A R144A K146A E148A | OB (B4) | Rpb4, Spt5_KOW3 | N | N | N | N | - |
| OB-9 | I143D 140–141Δ | OB (B4) | Rpb4 | N | 3 | N | N | 5 |
| OB-10 | I143D Q153Y L168Y A170Y | OB (B4, B4′, B6) | Rpb4, Spt5_KOW3 | N | N | 5 (3) | 30 (2) | N |
| OB-11 | R142A I143D R144A 167–171Δ | OB (B4, B6) | Rpb4 | - | 10 | 5 (0) | lethal | lethal |
| OB-12 | 151D I160D | OB (B4′, B5) | Spt5_KOW3 | - | 3 | N | lethal | lethal |
| OB-13 | Q153A H158A | OB (B4′, B5) | Spt5_KOW3, KOW4 | N | 3 | N | N | 8 |
| OB-14 | Q153Y | OB (B4′) | Spt5_KOW3, KOW4 | N | N | N | N | 8 |
| OB-15 | I157A | OB (B5) | ? | - | N | N | N | - |
| OB-16 | 161–171Δ | OB (B5-B6) | Rpb4 | lethal | lethal | lethal | lethal | lethal |
| OB-17 | 162–171Δ | OB (B5-B6) | Rpb4 | N | N | N (2) | lethal | 20 |
| OB-18 | 167–171Δ | OB (B6) | Rpb4 | N | N | N (0) | N | 10 |
a
Fold UV sensitivities were at 120, 40, 20, 40 and 6 J/m2 for WT, rad7Δ, rad7Δ rad26Δ, rad7Δ rad26Δ spt4Δ, and rad14Δ cells respectively. Numbers in parentheses indicate levels of TCR enhancement. N, UV sensitivity unchanged. -, untested.