FIG. 6.

CPE of recombinant BRSVs in BSR T7/5 cells (A) and MDBK cells (B). (A) In BSR T7/5 cultures, all recombinants induced syncytia of cells detaching from the monolayer, which were indistinguishable from those of standard ATue51908, at 56 h postinfection at an MOI of 0.01. (B) In MDBK cells, both full-length recombinant viruses rBRSV and rH/BRSV and standard ATue51908 virus produced similar foci of enlarged cells at 5 days postinfection, whereas the NS2 deletion mutants produced only small foci of degenerating cells.