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. 1999 Jan;73(1):270–280. doi: 10.1128/jvi.73.1.270-280.1999

FIG. 1.

FIG. 1

(A) Primer extension analysis of strain VR-2332. RNA from VR-2332-infected MA-104 cells was hybridized to γ-32P-radiolabeled VR-2332 leader reverse primer /658P4 and reverse transcribed. The primer extension products were electrophoresed alongside the known sequencing products obtained from clone 712 and forward primer P71/. The primer extension product migrated with the thymidine residue located at nucleotide 2965 of clone 712, resulting in an extension product of 98 nucleotides. (B) Comparison of PRRSV leader sequences. VR-2332 leader (190 bases in length) and LV leader (221 bases in length) sequences exhibit 61.0% identity as analyzed by the GCG GAP program, with a gap weight of 5 and a length weight of 5 (lines between the sequences indicate identity). The leader-body junction sequence utilized for transcription of each mRNA is boxed.

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