TABLE 3.
Comparison of the numbers of nucleotides between the leader-body junction site and the initiating AUG for sgmRNAs of VR-2332 (North American) and LV (European [37]) PRRSV
| ORF | mRNA | VR-2332 junction
|
No. of nucleotides
|
||
|---|---|---|---|---|---|
| Type | Sequencea | VR-2332 (no. of clones) | LVb | ||
| 2 | 2 | Leader | CACCCCUUuAACCAugUcUg | ||
| Subgenomic | CACCCCUUGAACCAACUUUA | 19 (5) | 38 | ||
| Genomic | CuguCaUUGAACCAACUUUA | ||||
| 3 | 3 | Leader | CACCCCUUUAACCAUgucug | ||
| Subgenomic | CACCCCUUUAACCAUAGUGU | 83 (4) | 11 | ||
| Genomic | guCaaaUgUAACCAUAGUGU | ||||
| 4 | 4.1 | Leader | CACCCCUUUaACCauGucUG | ||
| Subgenomic | CACCCCUUUCACCUAGAAUG | 4 (3) | 83 | ||
| Genomic | aAuuggUUUCACCUAGAAUG | ||||
| 4.2 | Leader | CACCCCUUuAaCCaUGUcUg | |||
| Subgenomic | CACCCCUUCAGCCGUGUUUC | 56 (1) | NDc | ||
| Genomic | CAaCauUUCAGCCGUGUUUC | ||||
| 5 | 5 | Leader | CACCCCUUUAaCCaugucUg | ||
| Subgenomic | CACCCCUUUAGCCUGUCUUU | 40 (3) | 32 | ||
| Genomic | aACuguUUUAGCCUGUCUUU | ||||
| 5-1 | Leader | CACCCCUUUAacCAugucug | |||
| Subgenomic | CACCCCUUUAGUCACUGUGU | 111 (1) | ND | ||
| Genomic | aguCuCUUUAGUCACUGUGU | ||||
| 6 | 6 | Leader | CACCCCUuUAACCAugucUg | ||
| Subgenomic | CACCCCUAUAACCAGAGUUU | 17 (4) | 24 | ||
| Genomic | aACCCCUAUAACCAGAGUUU | ||||
| 7 | 7.1 | Leader | CACCCCUUUAACCAuGucUg | ||
| Subgenomic | CACCCCUUUAACCACGCAUU | 123 (7) | 9 | ||
| Genomic | gcaaaugaUAACCACGCAUU | ||||
| 7.2 | Leader | CACCCCuUuAACCaUGUCug | |||
| Subgenomic | CACCCCGUAAACCUUGUCAA | 9 (3) | ND | ||
| Genomic | ggagugGUAAACCUUGUCAA | ||||
a
The leader-body junction motif for each sgmRNA is underlined. Nucleotides conserved between the subgenomic, leader, and genomic sequences are in uppercase.
b
Material reprinted with the permission of the Society of General Microbiology and J. J. M. Meulenberg (37).
c
ND, not detected.