Figure 4.

Low- (a) and high-dose (b) PTU-associated changes on the metabolome of the thyroid gland after short-term (2 weeks) and long-term drug administration (4 weeks), as well as an additional 2-week recovery phase after long-term exposure. Differences in global metabolome profiles between treatments (blue and red representing low and high doses, respectively) to controls (grey) were analyzed by PCA, with their significance determined by PERMANOVA. Differences in metabolite concentrations are visualized in a volcano plot depicting log2 fold changes of treatment versus control with their significance calculated by the Mann-Whitney U test and p-values corrected for multiple testing using the Benjamini-Hochberg method.