Table 2.
Results of the clinical sample analyzed using the one-step real-time TaqMan RT-PCR method, the analysis of HVRs at the vp2 gene, or reverse transcription PCR (RT-PCR) for the vp5 gene.
| Sample No. | One-Step Real-Time TaqMan RT-PCR | RT-PCR for vp5 Gene | HVRs of vp2 Gene | |
|---|---|---|---|---|
| FAM Cq | VIC Cq | |||
| S1 | negative | 20.23 | positive | nVarIBDV |
| S2 | 28.39 | negative | positive | non-nVarIBDV |
| S3 | 29.95 | negative | positive | N.D. |
| S4 | 32.25 | negative | positive | N.D. |
| S5 | 27.83 | negative | positive | non-nVarIBDV |
| S6 | 25.44 | negative | positive | non-nVarIBDV |
| S7 | 29.95 | negative | positive | N.D. |
| S8 | 24.01 | negative | positive | non-nVarIBDV |
| S9 | 26.63 | negative | positive | non-nVarIBDV |
| S10 | 27.22 | negative | positive | non-nVarIBDV |
| S11 | 25.54 | negative | positive | non-nVarIBDV |
| S12 | 28.67 | negative | positive | N.D. |
| S13 | negative | 22.28 | positive | nVarIBDV |
| S14 | 26.62 | negative | positive | non-nVarIBDV |
| S15 | 29.11 | negative | positive | N.D. |
| S16 | 27.55 | negative | positive | non-nVarIBDV |
| S17 | 26.71 | negative | positive | non-nVarIBDV |
| S18 | 28.95 | negative | positive | N.D. |
| S19 | 30.13 | negative | positive | N.D. |
| S20 | 25.46 | negative | positive | non-nVarIBDV |
| S21 | 27.02 | negative | positive | non-nVarIBDV |
| S22 | 25.18 | negative | positive | non-nVarIBDV |
| S23 | 22.93 | negative | positive | non-nVarIBDV |
| S24 | 27.36 | negative | positive | non-nVarIBDV |
| S25 | negative | 23.39 | positive | nVarIBDV |
| S26 | 26.49 | negative | positive | non-nVarIBDV |
| S27 | 28.16 | negative | positive | non-nVarIBDV |
| S28 | 25.77 | negative | positive | non-nVarIBDV |
| S29 | 26.32 | negative | positive | non-nVarIBDV |
| S30 | 29.15 | negative | positive | N.D. |
| S31 | 27.03 | 29.80 | positive | non-nVarIBDV |
| S32 | 21.43 | negative | positive | non-nVarIBDV |
| S33 | 25.75 | negative | positive | non-nVarIBDV |
| S34 | 23.13 | negative | positive | non-nVarIBDV |
| S35 | negative | 22.59 | positive | nVarIBDV |
| S36 | 29.10 | negative | positive | N.D. |
| S37 | 26.70 | negative | positive | non-nVarIBDV |
| S38 | 30.75 | negative | positive | N.D. |
| S39 | 26.60 | negative | positive | non-nVarIBDV |
| S40 | 29.02 | negative | positive | N.D. |
| S41 | 23.34 | negative | positive | non-nVarIBDV |
| S42 | 29.06 | negative | positive | N.D. |
| S43 | 22.77 | negative | positive | non-nVarIBDV |
| S44 | 26.68 | negative | positive | non-nVarIBDV |
| S45 | negative | 26.02 | positive | nVarIBDV |
| S46 | 26.33 | negative | positive | non-nVarIBDV |
| S47 | 27.21 | negative | positive | non-nVarIBDV |
| S48 | 25.83 | negative | positive | non-nVarIBDV |
| S49 | 25.96 | negative | positive | non-nVarIBDV |
| S50 | negative | 15.19 | positive | nVarIBDV |
| S51 | negative | 14.33 | positive | nVarIBDV |
| S52 | 22.24 | negative | positive | non-nVarIBDV |
| S53 | negative | 11.37 | positive | nVarIBDV |
| S54 | negative | 21.53 | positive | nVarIBDV |
| S55 | negative | 18.45 | positive | nVarIBDV |
| S56 | 27.71 | negative | positive | non-nVarIBDV |
| S57 | 32.14 | 29.23 | positive | N.D. |
| S58 | negative | 16.56 | positive | nVarIBDV |
| S59 | 24.82 | negative | positive | non-nVarIBDV |
| S60 | negative | 21.17 | positive | nVarIBDV |
| S61 | 23.13 | negative | positive | non-nVarIBDV |
| S62 | 25.93 | negative | positive | non-nVarIBDV |
| S63 | 26.15 | negative | positive | non-nVarIBDV |
| S64 | 28.01 | negative | positive | non-nVarIBDV |
| S65 | negative | 25.05 | positive | nVarIBDV |
| S66 | 27.97 | negative | positive | non-nVarIBDV |
| S67 | 27.44 | negative | positive | non-nVarIBDV |
| S68 | 31.07 | negative | positive | N.D. |
| S69 | 24.90 | negative | positive | non-nVarIBDV |
| S70 | negative | 23.50 | positive | nVarIBDV |
| S71 | 26.68 | negative | positive | non-nVarIBDV |
| S72 | 26.96 | negative | positive | non-nVarIBDV |
| S73 | 25.34 | negative | positive | non-nVarIBDV |
| S74 | 26.65 | negative | positive | non-nVarIBDV |
| S75 | 26.49 | negative | positive | non-nVarIBDV |
| S76 | 29.37 | negative | positive | N.D. |
| S77 | 25.38 | negative | positive | non-nVarIBDV |
| S78 | 28.48 | negative | positive | N.D. |
| S79 | negative | 18.21 | positive | nVarIBDV |
| S80 | negative | 18.58 | positive | nVarIBDV |
| S81 | 27.61 | negative | positive | non-nVarIBDV |
| S82 | 25.41 | negative | positive | non-nVarIBDV |
| S83 | 26.81 | negative | positive | non-nVarIBDV |
| S84 | negative | 21.48 | positive | nVarIBDV |