FIG. 3.

Expression of RLI and RNase L activity in H9 cells stably transfected with antisense and sense RLI cDNA. (A) Extracts (200 μg of proteins) from H9 cells stably transfected with the VV empty vector, with the sense RLI cDNA construction (VS1 and VS2), or with the antisense RLI cDNA construction (VAS1 and VAS2) were analyzed by SDS-PAGE and Western blotting with polyclonal antibody against RLI. (B) Cell extracts (600 μg of protein) from the different transfectants were tested without further fractionation in a 2-5A radiobinding assay. Results are expressed as the percentage of 2-5ApCp bound. 100% is the binding level in control VV cells.