TABLE 1.
Recombinant viruses with altered termini
| Recombinant | Recoverya | Burst sizeb (PFU/cell) | Plaque sizec (mm) |
|---|---|---|---|
| pVSV1(+) | + | 1,650 | 2.3 |
| pV1(+)3′CB | − | ||
| pV1(+)Le(TrC19–28) | − | ||
| pV1(+)Tr(LeC19–28) | + | 1,850 | 1.9 |
| pV1(+)Tr(LeC34–46) | + | 1,150 | 1.7 |
a
Mutations were incorporated into an infectious cDNA clone of VSV, pVSV1(+), using standard techniques, and attempts were made to recover infectious virus as described in Materials and Methods. +, infectious virus recovered; −, virus not recovered from at least six independent transfections.
b
Determined for growth in BHK-21 cells at 37°C.
c
Determined on BSC40 cells incubated at 37°C for 28 h.