TABLE 1.
Effects on RNA synthesis and assembly of deletions at the 5′ terminus of DI-T
DI | Replication (% DI-T)a | Minus strand (%)a | Plus strand (%)a | Assembly |
---|---|---|---|---|
DI-T | 100 | 56 | 44 | Yes |
DI/48 | 125 | 44 | 56 | Yes |
DI/47 | 125 | 46 | 54 | Yes |
DI/45 | 125 | 40 | 60 | Yes |
DI/36 | 36 | 37 | 63 | Yes |
DI/31 | 44 | 35 | 65 | Yes |
DI/29 | 46 | 31 | 69 | Yes |
DI/22 | ND | ND | ND | Low |
DI/14 | tr | ND | ND | No |
Average of two to four independent experiments using immunoprecipitated RNAs. Standard deviations ranged from 1.0 to 3.0. Not all of these DIs are represented in the autoradiograph shown in Fig. 6. ND, not determined.