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The typical calibration curve for NH4+ ion determinations. The calibration curve of NH4Cl standard solutions (r = 0.9891) generated through successive additions of NH4Cl in aqueous solutions that contain a final concentration of 20 mM HCl (final pH 2) and 10% (v/v) D2O. TSP-normalised integrals of the central line of the NH4+ ion signal were plotted as functions of the standard ammonium chloride concentration. Error bars indicate ± SEM values.
