FIG. 2.
Comparison of HIV-1 replication in PHA-PBMC, MT-4 cells, MDM cells, an IL-12-induced Th1-type culture, and an IL-4-induced Th2-type culture with molecular clones JR-CSF, JR-FL, and NL4-3 and chimeric viruses PHA-PBMC, MT-4 cells, Th1-type cells, and Th2-type cells (2 × 105) were infected with 200 TCID50 of virus. Thirty thousand MDM cells were infected with 30 TCID50 of virus. The amount of HIV-1 p24gag (nanograms/culture) in the culture medium was measured 11 days (PHA-PBMC, MT-4 cells, Th1-type cells, and Th2-type cells) and 21 days (MDM cells) after infection. Values represent the means and standard deviations from triplicate determinations. Results of one representative experiment with PHA-PBMC, MDM cells, Th1-type cells, and Th2-type cells from a single donor are shown. Similar levels of HIV-1 p24gag production were observed for the other three blood donors. The HIV-1 p24gag level in three primary cultures varied 1.2-fold for PHA-PBMC, 5.0-fold for MDM cells, 4.8-fold for Th1-type cells, and 18.6-fold for Th2-type cells.