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. 2023 Jul 15;28(14):5431. doi: 10.3390/molecules28145431

Table 3.

Processing influence on in vitro protein digestibility (IVPD). Improvement represented by ↑ and reduction by ↓.

Technology Conditions Results Reference
Baking Bread with 30% faba bean flour; faba bean sourdough bread ↑IVPD: 0.79–16.51 (%) [56]
Extrusion Temperature: (140–180 °C); moisture: 18–22%); unsoaked beans ↑IVPD: 3.71–4.26 (%) [57]
Temperature: (140–180 °C); moisture: 18–22%); soaked for 16 h at 30 °C ↑IVPD: 2.71–5.80 (%).
Extraction Cooked starch extract ↑Rapidly digestible starch increased by 475%
↓Slowly digestible starch reduced by 93%
↓Resistant starch reduced by 87%
[37]
Protein concentrate
Protein isolate
IVPD: 33.9
IVPD: 9.2
[34]
Protein isolate
Optimized ultrasound-treated isolate
IVPD: 68.42
IVPD: 65.98
[58]
Thermal treatment Extruded flour; cooking flour; baked flour ↓IVPD 2–4(%) [34]
Soaking Soaked flour in acidic and acidic + hydrogen peroxide
Soaked flour in water and neutral conditions
↑IVPD 11.78–14.75%
↓IVPD 2.0–7.4%
[59]
Time: 12–48 h at room temperature ↑IVPD 0.82–1.4% [26]
Thermal treatment Extrusion (barrel temperature between 30 and 120 °C); cooking; extrusion followed by baking for 27–35 min IVPD (%) was found to be 82.22, 81.41, and 76.79, respectively [60]
Cooking Soaked for 8 h followed by cooking ↑Albumin digestibility 29%
↑Glutelin digestibility 23.61%
[7]
Cooking (100 °C; 30 min); soaking + cooking (100 °C; 30 min); hulling + soaking + cooking (100 °C; 30 min) ↑IVPD 7.33–9.46 (%) [26]
Heat treatment Annealing with incubation at 65 °C for 24 h
Heat–moisture treatment; incubation at 120 °C for 24 h
↑IVPD 8.13 (%)
↓IVPD 4%
[55]
Fermented product Fermented and unfermented pasta formulation (10–50%). ↑IVPD 16.86–81% [61]
Bread with 50% faba flour
Bread with 50% fermented four using L. plantarum
IVPD 53.9%
IVPD 72.3%
[62]
Dehulling Hulled seeds ↑IVPD 0.79% [26]
Microwave Treatment Microwave for 6 min; soaking + microwave (6 min); hulling + soaking + microwave (6 min) ↑IVPD 1.75–3.5% [26]
Autoclaving Autoclaving (121 °C for 20 min) ↑IVPD 7.2–11% [26]
Irradiation Irradiated at 0.5 and 1 kGy
Irradiated at 0.5 and 1 KGy followed by cooking
↑IVPD 10–20%
↓IVPD 10–16%
[63]