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. 2023 Jul 20;28(14):5543. doi: 10.3390/molecules28145543

Figure 9.

Figure 9

The effects of different treatments on the immunohistochemical staining of Ki-67, as indicated by positive immune reactions (arrowheads), in the various groups studied (A–I). (A) A photomicrograph of a colon section immunostained with Ki-67; the normal colon of the control group demonstrates normal low nuclear Ki-67 expression. Scale bar = 200 μm, ×100. (B) A photomicrograph of an immunostained colon section with Ki-67 in the AP group reveals a normal low nuclear level of Ki-67 almost identical to that of the NC group. Scale bar = 200 μm, ×100. (C) A photomicrograph of an immunostained colon section with Ki-67 in the P group reveals a normal low nuclear level of Ki-67 that is nearly identical to the NC group. Scale bar = 200 μm, ×100. (D) A microphotograph of colon carcinoma induced via PHZ with Ki-67 immunostaining, revealing nuclear Ki-67 immunopositivity with abundant expression of inactive mutants Ki-67. Scale bar = 200 μm, ×100, (E) A photomicrograph of the PHZ + AP group immunostained with Ki-67 exhibited a lower level of Ki-67 expression than the PHZ group. Scale bar = 50 μm, ×400. (F) A photomicrograph of the PHZ + P group immunostained with Ki-67 revealed a lower level of Ki-67 expression in comparison to the PHZ group. Scale bar = 50 μm, ×400. (G) A photomicrograph of the combination of AP-P and PHZ immunostained with Ki-67 revealed a lower level of Ki-67 expression compared to the PHZ group. Scale bar = 50 μm, ×400. (H) A photomicrograph of the combined AP-P and PHZ (prophylaxis) simultaneously revealed a lower level of Ki-67 expression compared to the PHZ group. Scale bar = 50 m, ×400. (I) Ki-67 immunostaining intensity (percent area). The data are presented as means ± SEMs. *** and **** indicate significant differences (p < 0.001, and p < 0.0001).