Figure 2.

FC analysis of a tetravalent AH-adjuvanted vaccine prepared using a formulation strategy named separate adsorption: with this approach, each protein antigen (Ag) is individually adsorbed onto AH and the four monovalent samples are pooled together later. The four Ag used in this study are representative of different electrostatic strengths according to their isoelectric points (as reported in brackets). Overlay of the histograms at different time points shows the maturation of distribution of each single Ag over the AH particles: from highly polydispersed samples at T0 (dark red), to monodispersed samples after 130 days (light violet). In middle, samples after 30/50 days of incubation (light green) show maturation trend according to each electrostatic strengths: still polydispersed for Ag A, while already similar to sample after 130 days for Ag D. The X-axis indicates fluorescence intensity and the Y-axis number of events. All numbers refer to mean fluorescence intensity (MFI) values. From Reference [87].