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. 2023 Jul 4;16(7):960. doi: 10.3390/ph16070960

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative morphology images of RHN cells after treatment with different concentrations of the Precipitate M3 subfraction or BrainUp-10^®. Immunofluorescence assays represent different assay conditions. Control condition. Protein markers used in the experiment are shown as follows: Phalloidin: β-actin protein marker; α-Tubulin: microtubule-associated protein marker; Topro: cell core and merge of all the channels involved. The representative images of RHN present morphological changes after being treated with 0.5 μg/and mL; 1.0 μg/mL, 5.0 μg/mL of the Precipitate M3 subfraction or BrainUp-10^® for 24 h. DMSO is shown as the vehicle solution control.