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. 2023 Jul 4;16(7):960. doi: 10.3390/ph16070960

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative effect of AnSh fractions on the aggregation in vitro of recombinant hTau 40 protein. Concentrations of 5 μg/mL of each AnSh M3 subfraction, M2, and M3 fractions (individually or in combination), fulvic acid (FA), Andean Shilajit (AnSh), and BrainUp-10^® were tested (n = 1). (A) Representative tracing curves of aggregation of hTau 40 during 10 days of registration, with different treatments performed through Thioflavin S (ThS) fluorescence assay. (B) Representative images acquired by electron microscopy (magnification 25,000× g) of hTau 40 aggregation in the presence (+Hep, maximum) and absence (−Hep, minimum) of Heparin. (C) Inhibition percentage of hTau 40 aggregation obtained with different treatments. Highlighted are the values with the highest inhibition percentage.