Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Production of recombinant adenoviruses (rAdV): A shuttle vector containing the GOI is recombined with a plasmid containing adenoviral genes but lacking E1 and E3 (pAd). The resulting pAd-GOI is transfected into packaging cells that express E1A and allow replication of rAdV. Ultracentrifugation with CsCl (cesium chloride) is used to separate rAdV from cellular debris and finally dialyzed to obtain purified rAdV.
