Figure 4.
(A) Representative Bright-field images obtained by Optical Microscopy of senescent and non-senescent Human dermal fibroblasts (HDF) after scrape, upon incubation with the formulations. Definition of region of interest (ROI–red line) for % wound closure definition. (B) % wound closure after 3, 7, 24 h of incubation with formulations. Abbreviations: CTR: control; HA: hyaluronic acid; HDF: human derived fibroblasts; SLN: solid lipid nanoparticles. All results are presented as mean ± standard deviation. For HDF experimental set: * p value < 0.0001 vs. SLN, **** p value < 0.0001 vs. CTR (24 h); * p value < 0.0001 vs. SLN, ** p value < 0.0001 vs. HA, **** p value < 0.0001 vs. CTR (7 h); **** p value < 0.0001 vs. HA; * p value < 0.0001 vs. CTR (3 h). For senescent HDF experimental set: **** p value < 0.0001 vs. SLN, ** p value < 0.0001 vs. HA, **** p value < 0.0001 vs. CTR (24 h); * p value < 0.0001 vs. SLN, * p value < 0.0001 vs. HA, **** p value < 0.0001 vs. CTR (7 h).