Table 4.

Dynamic Light Scattering (DLS) particle size characterization of blank and 6-cum loaded solid lipid nanoparticles (SLNs) purified by either size exclusion chromatography or dextran-gradient centrifugation and resuspension. Abbreviations: 6-cum: 6-coumarin; Cp: cholesteryl palmitate; Hh: hexyl hexadecanoate; N.D.: not determined (SLNs not formed or exceeding DLS detection capability): PDI: polydispersion index; SLNs: solid lipid nanoparticles; Sp: span; Tm: trimystirin; Tp: tripalmitin; Tw20: tween 20. Statistical analysis: 6-cum SLNs purified by size exclusion vs. blank SLNs purified by size exclusion p < 0.005 *; p < 0.001 **; p < 0.0005 ***; p < 0.0001 ****. 6-cum SLNs purified by dextran-gradient centrifugation and resuspension vs. 6-cum SLNs purified by size exclusion p < 0.0005 ^§§§; p < 0.0001 ^§§§§.

SLNs	Blank SLNs Purified by Size Exclusion		6-cum SLNs Purified by Size Exclusion		6-cum SLNs Purified by Dextran-Gradient Centrifugation and Resuspension
	Mean Size (nm)	PDI	Mean Size (nm)	PDI	Mean Size (nm)	PDI
2% Tp 4% Sp60 8% Tw20	711.4 ± 50.8	0.327	255.9 ± 7.1 **	0.293	293.9 ± 6.2	0.256
2% Tp 2% Sp60 5% Tw20	N.D.	N.D.	168.5 ± 3.3	0.237	139.8 ± 35.9	0.281
2% Tm 4% Sp60 8% Tw20	202.2 ± 4.0	0.159	75.1 ± 2.0 ****	0.255	219.7 ± 6.3 §§§§	0.248
2% Tm 2% Sp60 5%Tw20	N.D.	N.D.	170.1 ± 0.1	0.184	469.3 ± 138.4	0.284
2% Cp 4% Sp60 8% Tw20	210.8 ± 4.6	0.204	64.4 ± 0.9 ****	0.228	213.2 ± 3.2 §§§§	0.219
2% Cp 2% Sp60 5% Tw20	579.3 ± 26.6	0.228	236.0 ± 3.4 *	0.220	347.5 ± 82.1	0.203
2% Hh 4% Sp60 8% Tw20	71.9 ± 0.9	0.153	46.8 ± 1.8 ***	0.309	145.6 ± 36.7	0.463
2% Hh 2% Sp60 5% Tw20	315.8 ± 4.1	0.088	98.8 ± 1.8 ****	0.251	122.8 ± 2.9	0.290
2% Hh 4% Sp40 8% Tw20	100.5 ± 0.8	0.132	56.6 ± 0.5 ****	0.219	190.3 ± 3.1 §§§§	0.331
2% Hh 4% Sp80 8% Tw20	71.2 ± 3.6 ****	0.266	132.7 ± 1.5	0.184	233.7 ± 6.7 §§§	0.257
2% Hh 2% Sp80 5% Tw20	296.1 ± 0.9	0.151	192.6 ± 1.7 ****	0.197	210.9 ± 15.4	0.252
2% Hh 4% Sp80 5% Tw20	91.0 ± 1.5	0.127	84.1 ± 1.1	0.251	250.2 ± 8.4 §§§§	0.291