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Restricted expression of HIV-1 p24 during acute-phase viral replication in primary and transformed astrocytes compared with HeLa cells. The expression of HIV-1 p24 or the GFP reporter is shown from HeLa, U251MG astrocytoma, or PFA cells that were cotransfected with the pNL4-3 proviral plasmid and pEGFP-N1. Cells and supernatant were tested at their peak of expression, 48 h for HeLa cells and 72 h for both astrocytic cell types.
