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. 1999 Jan;73(1):352–361. doi: 10.1128/jvi.73.1.352-361.1999

FIG. 2.

FIG. 2

Efficient transcription and nucleocytoplasmic transport of HIV-1 mRNA in astrocytes. Northern blotting, using a 3′ HIV-1 LTR region probe designed to detect all HIV-1 mRNAs, was performed with total cellular RNA from HeLa (lanes 1 and 2) and U251MG cells (lanes 3 and 4) transfected with pNL4-3 (lanes 2 and 4) or mock transfected (lanes 1 and 3) (A) or with cytoplasmic (C) or total (T) RNA extracted from HeLa (lanes 1 to 4) and U251MG (lanes 5 to 8) cells transfected with pNL4-3 (lanes 3, 4, 7, and 8) or mock transfected (lanes 1, 2, 5, and 6) (B) or with cytoplasmic or total RNA extracted from HeLa (lanes 1 to 4) and U251MG (lanes 5 to 8) cells transfected with pNL4-3 Rev− (lanes 3, 4, 7, and 8) or mock transfected (lanes 1, 2, 5, and 6) (C).