FIG. 8.

Efficient translation of a non-HIV-1 fluorescence reporter by astrocytes. Flow cytometry profiles showing the transfection-translation efficiency of GFP (light shading) by U251MG astrocytes (A and C) and HeLa cells (B and D) transfected with 5 μg of pEGFP-N1 alone (A and B) or together with 20 μg of the HIV-1 proviral plasmid pNL4-3 (C and D). FACS analysis was performed at the peak of expression posttransfection (72 h for U251MG and 48 h for HeLa cells). Mock transfections (dark shading) contained either no DNA (A and B) or pNL4-3 alone (C and D). The region R1 shows the percentage and the mean channel fluorescence (MCF) of cells expressing GFP above the mock-transfection values.