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Nuclear export of 9-kb Rev-dependent HIV-1 mRNA
| Cell line | Plasmid transfected | Cytoplasmic mRNA (% of total)a |
|---|---|---|
| HeLa | pNL4-3 | 26 |
| U251MG | pNL4-3 | 24 |
| HeLa | pNL4-3Rev− | 0.8 |
| U251MG | pNL4-3Rev− | 0.5 |
The percent accumulation of 9-kb HIV-1 mRNA detected in the cytoplasmic fraction relative to that in the total-cell RNA of astrocytes and HeLa cells transfected with pNL4-3 and pNL4-3Rev− (Fig. 2B and C) was determined from densitometric analysis of Northern blot pNL4-3Rev− assays.
