Table 1.
Strains used in this study (E. coli). They contain the template plasmids or the newly developed acceptor chromogenic expression vectors. The suffix ‘-ex’ for the marker indicates the presence of LoxR/LoxP motifs that are excisable using a Cre-lox recombination method (Fickers et al. 2003).
| Plasmid name | Description | Purpose | Reference |
|---|---|---|---|
| JME1046 | JMP62-URA3ex-pTEF | Backbone and URA3 gene conversion | (Nicaud et al. 2002) |
| JME2563 | JMP62-LEU2ex-pTEF | Backbone and LEU2 gene conversion | (Le Coq, unpublished) |
| JME3265 | JMP62-LYS5ex | Backbone and LYS5 gene conversion | (Park et al. 2019b) |
| GGE0449 | pSB1A3-URA3ex-Tlip2-AmilCP-Txpr2 | AmilCP fragment | (Vidal et al. 2023) |
| GGE0114 | pSB1A3-URA3ex-RFP | RFP amplification | (Celińska et al. 2017) |
| JME4861 | TOPO-pEYL1 | pEYL1 amplification | (Vidal et al. 2023) |
| JME4890 | TOPO-pEYL1-5AB | pEYL1-5AB amplification | (Vidal et al. 2023) |
| JME4243 | JMP62-URA3ex-pHU8EYK-CalB | pHU8EYK fragment | (Park et al. 2019b) |
| JME4365 | JMP62-URA3ex-pEYK1-3AB-CalB | pEYK1-3AB fragment | (Park et al. 2019b) |
| JME2471 | JMP62-LEU2ex-pHp4d-RedStarII | pHp4d fragment | (Dulermo et al. 2017) |
| JME5568 | JME1046 in dam− E. coli strain | prevent ClaI methylation | This study |
| JME5569 | JME3265 in dam− E. coli strain | prevent ClaI methylation | This study |
| JME5570 | JME4243 in dam− E. coli strain | prevent ClaI methylation | This study |
| GGE0440 | CRISPR-Cas9-LYS5ex-sgRNA-MHY1 | MHY1 gene deletion | (Lebrun, unpublished) |
| JME5574 | JMP62-URA3ex-pTEF-RFP | chromogenic expression vector | This study |
| JME5781 | JMP62-URA3ex-pHp4d-RFP | chromogenic expression vector | This study |
| JME5576 | JMP62-URA3ex-pHU8EYK-RFP | chromogenic expression vector | This study |
| JME5577 | JMP62-URA3ex-pEYK1-3AB-RFP | chromogenic expression vector | This study |
| JME5578 | JMP62-URA3ex-pEYL1-RFP | chromogenic expression vector | This study |
| JME5579 | JMP62-URA3ex-pEYL1-5AB-RFP | chromogenic expression vector | This study |
| JME5599 | JMP62-LYS5ex-pTEF-AmilCP | chromogenic expression vector | This study |
| JME5601 | JMP62-LYS5ex-pHp4d-AmilCP | chromogenic expression vector | This study |
| JME5602 | JMP62-LYS5ex-pHU8EYK-AmilCP | chromogenic expression vector | This study |
| JME5782 | JMP62-LYS5ex-pEYK1-3AB-AmilCP | chromogenic expression vector | This study |
| JME5783 | JMP62-LYS5ex-pEYL1-AmilCP | chromogenic expression vector | This study |
| JME5784 | JMP62-LYS5ex-pEYL1-5AB-AmilCP | chromogenic expression vector | This study |
| JME5785 | JMP62-LEU2ex-pTEF-RFP | chromogenic expression vector | This study |
| JME5575 | JMP62-LEU2ex-pHp4d-RFP | chromogenic expression vector | This study |
| JME5786 | JMP62-LEU2ex-pHU8EYK-RFP | chromogenic expression vector | This study |
| JME5666 | JMP62-LEU2ex-pEYK1-3AB-RFP | chromogenic expression vector | This study |
| JME5668 | JMP62-LEU2ex-pEYL1-RFP | chromogenic expression vector | This study |
| JME5670 | JMP62-LEU2ex-pEYL1-5AB-RFP | chromogenic expression vector | This study |