Table 4.
Expression vectors constructed during this study. Vectors contain the RFP or the AmilCP gene to produce the chromoprotein resulting in red or blue E. coli colonies, respectively. The suffix ‘-ex’ for the marker indicates the presence of LoxR/LoxP motifs that are excisable using a Cre-lox recombination method (Fickers et al. 2003).
| Promoter | Characteristics | URA3ex | LYS5ex | LEU2ex |
|---|---|---|---|---|
| pTEF | Constitutive | red | blue | red |
| pHp4d | Phase-dependent, 4 copies UASxpr2-coreLEU2 | red | blue | red |
| pHU8EYK | Erythritol-inducible, 8 copies UASxpr2-coreEYK1 | red | blue | red |
| pEYK1-3AB | Erythritol-inducible, 3 copies UASeyk1-pEYK1 | red | blue | red |
| pEYL1 | Erythritol-inducible | red | blue | red |
| pEYL1-5AB | Erythritol-inducible, 5 copies UASeyk1-pEYL1 | red | blue | red |