Colocalization of HSV-gE with TGN proteins. HeLa cells double transfected with mammalian expression vectors encoding HSV-gE and either VZV-gE (a and b), furin (c and d), or ST-VSVG (e and f) were visualized with the 7520 MAb against HSV-gE (a, c, and e) and the 1667 polyclonal antiserum against VZV-gE (b), a polyclonal serum against furin (d), and a polyclonal antiserum against the VSVG epitope (f), followed by fluorescein isothiocyanate- or rhodamine-coupled antirabbit and antimouse secondary antibodies. Bar, 5 μm.