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Effect of BFA treatment on the subcellular distribution of HSV-gE. HeLa cells were double transfected with pSFFV-HSV-gE and either pSFFV-VZV-gE (a and b) or pSG5-furin (c and d) and treated for 5 min with 10 μg of BFA per ml. After fixation, the coverslips were processed for indirect immunofluorescence to detect HSV-gE (7520 MAb) (a and c) and VZV-gE (1667 polyclonal antiserum) (b) or furin (d). Bar, 5 μm.
