FIG. 4.

Recycling of HSV-gE through the plasma membrane. HeLa cells were double transfected with plasmids encoding VZV-gE and either HSV-gE (a and b) or HSV-gEΔ1 (c and d) and 48 h after transfection were incubated for 1 h at 37°C in culture medium containing 1:200 dilutions of the 1667 polyclonal rabbit antiserum against VZV-gE and the 7520 MAb against HSV-gE. Cells were fixed and processed for immunofluorescence by using a mixture of fluorescein isothiocyanate-coupled antirabbit IgG (to detect anti-VZV-gE [a and c]) and rhodamine (tetramethyl rhodamine isothiocyanate)-coupled antimouse IgG (to detect anti-HSV-gE [b and d]). Bar, 5 μm.