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Intracellular distribution of HSV-gE mutants in the cytoplasmic domain. HeLa cells were double transfected with a plasmid encoding VZV-gE and either HSV-gEΔ4 (a and b), the HSV-gEΔ3 (c and d), the HSV-gEΔ2 (e and f), or the HSV-gEΔ1 (g and h) truncation mutants or the HSV-gE(Y463A) (i and j) point mutant and stained with a mixture of the 1667 polyclonal antiserum against VZV-gE (a, c, e, g, and i) and the 7520 MAb against HSV-gE (b, d, f, h, and j). Bar, 5 μm.
