Table 2.
ADE assays performed using the different cell lines, viruses, or pseudo-typed viruses.
| Virus Studied | Cell Line | Pseudovirus | Reporter System | Readout | References |
|---|---|---|---|---|---|
| DENV | K562 and Mylc cells. | Single-round infection particles (SRIPs). | Luciferase. | Luminometer. | [60] |
| Ebola | Human embryonated kidney (293) cells (HEK293) or Vero E6 | Pseudotyped vesicular stomatitis virus (VSV) and VSVΔG-EBOV GP. | GFP. | Fluorescent microscopy. | [11] |
| HIV | CD21-expressing cell line and T cell line naturally expressing complement receptor 2 (CR2; CD21). | HIV isolates and simian-human immunodeficiency viruses (SHIVs). |
Intracellular staining for P24 expression. | Flow cytometry. | [62] |
| SARS-CoV-2 | Raji cells, K562 cells, primary B cells, and Vero E6. | Spike protein expressing pseudovirus and VSV pseudotyped with SARS CoV-2 S (VSV-SARS2). | Luciferase and GFP. | Luminometer, flow cytometry, and fluorescent microscopy. | [20] |