TABLE 1.

Recognition of truncations of the dengue type 4 virus NS3 peptide (residues 296 to 310) by CD8⁺ CTL clone 2D65^a

Expt	Peptide residues	Sequence	% Specific lysis at peptide concn of:
			5 μM	500 nM	50 nM	5 nM
1	296–310	ARGYISTRVEMGEAA	56	23	3	2
	298–310	GYISTRVEMGEAA	58	55	36	10
	300–310	ISTRVEMGEAA	1	2	2	2
	296–308	ARGYISTRVEMGE	58	34	6	5
	296–306	ARGYISTRVEM	59	32	5	3
2	298–306	GYISTRVEM	25	27	27	18
	298–305	GYISTRVE	6	3	11	5
	299–306	YISTRVEM	21	19	18	9
	300–306	ISTRVEM	9	2	4	9

^aRecognition of synthetic peptide-pulsed target cells was tested at effector/target cell ratios of 4:1 in experiment 1 and 2:1 in experiment 2. Percentages of lysis of target cells in the absence of peptide were 2% in experiment 1 and 9% in experiment 2. Boldface values represent percent specific lysis significantly greater than that of the negative control.