TABLE 4.
Serotype cross-reactivity of NS3-specific CTLs in dengue type 3 virus-immunized micea
| Day of culturing | Target cellsb | % Specific lysis |
|---|---|---|
| 27 | D4-NS3 (298–306) | 25 |
| D3-NS3 (299–307) | 27 | |
| WNV NS3 (299–307) | 4 | |
| 40 | D2V | 57 |
| Uninfected | −2 | |
| vvD2:NS3 | 31 | |
| vvD3:NS3 | 43 | |
| vvD4:NS3 | 25 | |
| vvControl | 1 |
Spleen cells from mice immunized with dengue type 3 virus were stimulated in vitro with the dengue type 3 virus NS3 (D3-NS3) peptide (residues 299 to 307) and tested for recognition of the indicated target cells. Boldface values represent percent specific lysis significantly greater than that of the negative control.
P815 target cells were incubated with the corresponding peptides of dengue type 4 virus, dengue type 3 virus, or WNV (day-27 effectors) or were infected with dengue type 2 virus (D2V), wild-type vaccinia virus (vvControl), or recombinant vaccinia viruses (vv) expressing the dengue type 2, dengue type 3, or dengue type 4 virus NS3 proteins (day-40 effectors) (e.g., vvD2:NS3 represents dengue type 2 virus NS3 protein expressed by a recombinant vaccinia virus).