Fig. 3.

CD44⁺ CSCs-treated with LC at a dose of 2.5 and 5 mM enhanced apoptosis rate. After 24 h of treatment, CD44⁺ CSCs were stained with Annexin V/PI assay followed by flow cytometry analysis. A shift from bottom-right quadrant panel (early apoptosis) to top-right quadrant panel (late apoptosis) and top-left quadrant panel (necrosis) was observed. (A) A total population of CD44⁺ CSCs; (B) Unstained cells; (C) CD44⁺ CSCs in the absence of LC; (D) CD44⁺ CSCs treated with 2.5 mM LC; (E) CD44⁺ CSCs treated with 5 mM LC. The quantification of apoptosis was shown in part F. Values are mean ± SD from independent experiments (∗P < 0.05 and ∗∗∗∗P < 0.0001, n = 3).