Figure 5.

The targeted complexes were able to identify both localized and metastatic cancer cells in xenograft pancreatic cancer mouse models with renal capsule tumors (n = 1 per treatment group). (A) Quantified bioluminescent intensities in various organs for each mouse treatment. (B) Quantified bioluminescent intensities in the tumor normalized to the tumor volume. (C–E) Bioluminescent images of the various organs for each mouse treatment. Bioluminescent signal was only detected in the lungs and the right kidney, where the tumor grew, for the mouse that received the targeted complex, while the nontargeted complex had high signals in the right kidney, lungs, heart, and intestines. The targeted protein had slight off-target signals. (F–H) H&E-stained tissue slices of the lungs (left image) and right kidneys (right image) from the organs in (B) for the fusion protein, targeted complex, and control, respectively. Metastatic pancreatic cancer cells were identified through the darker purple tissues. (I–K) IVIS bioluminescent images of the tumor sites prior to sacrifice for the mice treated with the fusion protein, targeted complex, and control, respectively. (L–N) Visualization of the three treatments used, indicating which treatment was represented in each column.