Table 2.
Effect of alanine scan on SARS-CoV-2 inhibitory activity of peptide PN19
| Peptide | Sequence | IC50 (mean ± SD) μM | CC50 (mean ± SD) μM |
|---|---|---|---|
| PN19wt | 888FGAGAALQIPFAMQMAYRF906 | 0.80 ± 1.68 | > 100 |
| PN19F→A888 | 888AGAGAALQIPFAMQMAYRF906 | 2.91 ± 1.95 | > 100 |
| PN19L→A894 | 888FGAGAAAQIPFAMQMAYRF906 | 0.60 ± 0.42 | > 100 |
| PN19Q→A895 | 888FGAGAALAIPFAMQMAYRF906 | 3.82 ± 3.50 | >100 |
| PN19I→A896 | 888FGAGAALQAPFAMQMAYRF906 | 0.30 ± 1.40 | > 100 |
| PN19P→A897 | 888FGAGAALQIAFAMQMAYRF906 | 0.24 ± 0.14 | > 100 |
| PN19F→A898 | 888FGAGAALQIPAAMQMAYRF906 | 57.73 ± 66.17 | > 100 |
| PN19M→A900 | 888FGAGAALQIPFAAQMAYRF906 | 0.84 ± 0.52 | > 100 |
| PN19Q→A901 | 888FGAGAALQIPFAMAMAYRF906 | 3.10 ± 0.82 | > 100 |
| PN19M→A902 | 888FGAGAALQIPFAMQAAYRF906 | 1.41 ± 2.42 | > 100 |
| PN19Y→A904 | 888FGAGAALQIPFAMQMAARF906 | 28.32 ± 47.96 | > 100 |
| PN19R→A905 | 888FGAGAALQIPFAMQMAAAF906 | 0.85 ± 0.72 | > 100 |
| PN19F→A906 | 888FGAGAALQIPFAMQMAYRA906 | 2.65 ± 2.45 | > 100 |
aAla scan was performed in all amino acid residues of PN19 except for Ala and Gly residues of wild type sequence. Values obtained in two to three independent assays.