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. 2023 Jul 13;12(7):934. doi: 10.3390/pathogens12070934

Table 1.

Primers used in the study for diagnosis and sequencing of rotavirus C in the samples.

Primers Diagnostic, Genotyping, and Sequencing Primer Annealing Temperature (°C) Amplicon Size (bp) References
RVC VP6-F & RVC VP6-R 5′-GGCTTTAAAAATCTCATTCA-3′ 48 1353 bp [34]
3′-AGCCACATAGTTCACATTTC-5′
RVC-VP6-PF & BMJ44 5′ARTCHGTTCTATGYGATTC3′ 48 335 [31,33]
3′AGCCACATAGTTCACATTTC5′
RVC-VP6-PF & RVC-VP6-PR 5′TCATACTGGGGCATTGGAAC3′ 48 584 [31]
3′GCCAAGTGTTTGATTATTAGG5′
GrC VP7-20F & GrC VP7-1062R 5′GCTGTCTGACAAACTGGTC3′ 48 1241 [13]
3′GCCACATGATCTTGTTTACGC5′
RVC-NSP4-CDS-FP & RVC-NSP4-CDS-RP 5′CTCTACGAAGCAATGGAGTTCATCAA3′ 48 477 [31]
3′AGCGCAGAAGATTCATAGACA5′