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. 2023 Jun 29;16(7):946. doi: 10.3390/ph16070946

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mitochondria and cytochrome c immunostaining in MDA-MB-231 cells. In DMSO-treated cells, cytochrome c (panel (C), CTRL) resides within mitochondria (panel (B), CTRL), as visible in the overlay channel (panel (D), CTRL). MCF-7 cells treated with 7e (concentration equal to its IC₅₀ value, for 24 h) show cytochrome c release in the cytosol (panel (C), 7e) and changes in mitochondrial structure (panel (B), 7e). (A) Nuclear stain with DAPI (excitation/emission wavelength 350 nm/460 nm). (B) MitoTracker Deep Red FM probe (excitation/emission wavelength 644 nm/665 nm). (C) Alexa Fluor CF 488 (excitation/emission wavelength 490 nm/515 nm). (D) Merge channels. Images were taken at 40× magnification, and representative fields are shown.