. 2023 Jul 18;28(14):5485. doi: 10.3390/molecules28145485

Table 1.

Design matrix in the Box–Behnken model and responses (represented with SD values for each response) for green tea extracts (total catechins represents all the catechins including EGCG).

Exp N°	Coded Levels			Results (Concentration mg/g of Biomass after 45 min of Extraction)									Total Molecules (mg/g of Biomass)
	X₁	X₂	X₃	Caff	GC	EGC	Cat	EGCG	EC	ECG	Total Catechins	Total Molecules (Y mg/g)	Fraction 1 (15 min)	Fraction 2 (30 min)	Fraction 3 (45 min)
	Temperature (°C)	Modifier (%)	H₂O in Modifier (%)	Caff	GC	EGC	Cat	EGCG	EC	ECG	Total Catechins	Total Molecules (Y mg/g)	Fraction 1 (15 min)	Fraction 2 (30 min)	Fraction 3 (45 min)
1	40 (−1)	10 (−1)	10 (0)	13.04 ± 1.01	0.18 ± 0.01	0.09 ± 0.01	0.04 ± 0.00	0.12 ± 0.01	0.31 ± 0.02	0.12 ± 0.01	0.86 ± 0.06	13.90 ± 0.93	8.94 ± 1.19	3.68 ± 0.66	1.28 ± 0.20
2	80 (+1)	10 (−1)	10 (0)	13.13 ± 1.01	0.46 ± 0.02	2.46 ± 0.13	0.14 ± 0.01	4.53 ± 0.43	1.34 ± 0.08	1.88 ± 0.11	10.80 ± 0.72	23.93 ± 1.60	9.16 ± 1.22	3.42 ± 0.62	11.36 ± 1.75
3	40 (−1)	30 (+1)	10 (0)	17.24 ± 1.33	1.50 ± 0.05	14.40 ± 0.76	0.55 ± 0.03	22.75 ± 2.18	5.27 ± 0.30	6.39 ± 0.38	50.87 ± 3.40	68.11 ± 4.55	42.63 ± 5.69	15.52 ± 2.80	9.96 ± 1.53
4	80 (+1)	30 (+1)	10 (0)	19.75 ± 1.53	2.14 ± 0.08	20.35 ± 1.07	0.78 ± 0.04	32.84 ± 3.14	7.08 ± 0.40	9.79 ± 0.58	72.98 ± 4.88	92.73 ± 6.20	74.51 ± 9.94	12.14 ± 2.19	6.08 ± 0.93
5	40 (−1)	20 (0)	0 (−1)	3.59 ± 0.28	0.11 ± 0.00	0.94 ± 0.05	0.05 ± 0.00	1.17 ± 0.11	0.54 ± 0.03	0.57 ± 0.03	3.38 ± 0.23	6.97 ± 0.47	4.93 ± 0.66	1.15 ± 0.21	0.89 ± 0.14
6	80 (+1)	20 (0)	0 (−1)	9.42 ± 0.73	0.22 ± 0.01	1.55 ± 0.08	0.12 ± 0.01	2.07 ± 0.20	1.14 ± 0.07	0.99 ± 0.06	6.10 ± 0.41	15.52 ± 1.04	9.60 ± 1.28	3.29 ± 0.59	2.63 ± 0.40
7	40 (−1)	20 (0)	20 (+1)	16.78 ± 1.30	1.99 ± 0.07	19.13 ± 1.01	1.19 ± 0.05	32.07 ± 3.07	6.46 ± 0.37	9.57 ± 0.56	70.41 ± 4.70	87.19 ± 5.83	58.42 ± 7.79	23.67 ± 4.27	5.10 ± 0.78
8	80 (+1)	20 (0)	20 (+1)	13.56 ± 1.05	2.12 ± 0.08	14.56 ± 0.77	1.40 ± 0.06	20.72 ± 1.98	6.27 ± 0.36	8.23 ± 0.48	53.30 ± 3.56	66.86 ± 4.47	55.97 ± 7.47	9.20 ± 1.66	1.70 ± 0.26
9	60 (0)	10 (−1)	0 (−1)	10.45 ± 0.81	0.03 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.03 ± 0.00	10.49 ± 0.70	8.80 ± 1.17	1.05 ± 0.19	0.64 ± 0.10
10	60 (0)	30 (+1)	0 (−1)	6.37 ± 0.49	0.34 ± 0.01	3.29 ± 0.17	0.15 ± 0.01	4.30 ± 0.41	1.34 ± 0.08	1.43 ± 0.08	10.85 ± 0.72	17.22 ± 1.15	10.44 ± 1.39	4.07 ± 0.73	2.71 ± 0.42
11	60 (0)	10 (−1)	20 (+1)	13.77 ± 1.06	1.58 ± 0.06	11.62 ± 0.61	1.06 ± 0.05	15.33 ± 1.47	4.71 ± 0.27	6.44 ± 0.38	40.74 ± 2.72	54.51 ± 3.64	12.90 ± 1.72	26.67 ± 4.81	14.94 ± 2.30
12	60 (0)	30 (+1)	20 (+1)	17.95 ± 1.39	2.00 ± 0.07	20.44 ± 1.08	1.33 ± 0.06	31.55 ± 3.02	6.88 ± 0.39	9.95 ± 0.59	72.15 ± 4.82	90.10 ± 6.02	85.10 ± 11.35	3.90 ± 0.70	1.11 ± 0.17
13	60 (0)	20 (0)	10 (0)	16.02 ± 1.24	1.66 ± 0.06	14.93 ± 0.79	0.63 ± 0.03	20.70 ± 1.98	5.68 ± 0.32	7.28 ± 0.43	50.89 ± 3.40	66.91 ± 4.47	45.64 ± 6.09	12.04 ± 2.17	9.23 ± 1.42
14	60 (0)	20 (0)	10 (0)	16.91 ± 1.31	1.76 ± 0.06	15.47 ± 0.82	0.68 ± 0.03	20.87 ± 2.00	6.49 ± 0.37	8.04 ± 0.47	53.32 ± 3.56	70.23 ± 4.69	43.18 ± 5.76	14.63 ± 2.64	12.42 ± 1.91
15	60 (0)	20 (0)	10 (0)	17.74 ± 1.37	1.75 ± 0.06	15.33 ± 0.81	0.66 ± 0.03	20.64 ± 1.98	6.29 ± 0.36	7.89 ± 0.46	52.55 ± 3.51	70.29 ± 4.70	45.53 ± 6.07	14.66 ± 2.64	10.11 ± 1.55
16	60 (0)	20 (0)	10 (0)	19.70 ± 1.52	1.83 ± 0.07	16.76 ± 0.88	0.70 ± 0.03	25.01 ± 2.39	6.58 ± 0.38	8.56 ± 0.50	59.43 ± 3.97	79.13 ± 5.29	55.41 ± 7.39	15.46 ± 2.79	8.26 ± 1.27
17	60 (0)	20 (0)	10 (0)	17.85 ± 1.38	1.72 ± 0.06	14.63 ± 0.77	0.63 ± 0.03	19.83 ± 1.90	6.12 ± 0.35	7.74 ± 0.46	50.67 ± 3.39	68.52 ± 4.58	38.77 ± 5.17	19.64 ± 3.54	10.11 ± 1.55