FIG. 5.

Coimmune precipitation of HAUSP with Vmw110 from extracts of cells infected with wild-type and Vmw110 substitution mutant viruses. An experiment similar to that illustrated in Fig. 5 was conducted with wild-type virus positive control (1–775), the mutant E52X negative control (1–593), and the substitution-carrying mutant viruses M1, M2, and M4. The upper part of the figure shows proteins detected by Western blotting by using anti-HAUSP serum r201 in a sample of cell extract and in anti-Vmw110 immune precipitates from mock-infected (mock) and virus-infected cells as indicated. The arrow indicates the position of HAUSP. The lower part shows the presence of Vmw110 proteins in the immune precipitates after reprobing of the filter with anti-Vmw110 serum r95.