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Time course of accumulation of R3Z RNA following transfection of KJT7 cells with T7R3Z DNA. RNase protection experiments show the amount of positive-strand R3Z RNA at various times posttransfection (see Materials and Methods). The amount of R3Z RNA in extracts prepared from 4 × 106 KJT7 cells was determined from standard curves of the RNase protection signal from known amounts of R3Z RNA transcripts.
